부산대학교 도서관

The objective of this research program is to develop a liver tissue to be employed in a hepatic assist device for metabolic activity of the liver.Two forms of the liver tissue, slice and cube, were first compared. As for incubation medium, perfluorocheaical emulsion was employed with ar without tissue culture media. Urea production rate was a major indicator to assess metabolic activity of the tissue.The result indicated that i) the liver tissue in slice form yields higher activity than that in cubic form, ii) the liver slice actively utilize loaded aarmonia, and iii) although the perfluorochaical enulsion alone can maintain the viability of the liver tissue, its combination with tissue culture media further enhance the metabolic activity. Viability of the tissue was maintained up to 20 hours or more and over 24 hours' maintenance appeared possible.It is believed that the liver tissue thus prepared could be incorporated in “An artificial liver” in terms of metabolic replac anent.