부산대학교 도서관

Rats with bladder tumor induced by BBN were treated by intravesical instillation of 0.8mg of (2″R)-4′-O-tetrahydropyranyladriamycin (THP) (9rats) or adriamycin (ADM) (8rats) dissolved in 0.2ml of distilled water. Thirty minutes later, the bladder was removed surgically. Rats without the tumor received the same treatment of THP (8rats) or ADM (9rats). THP and ADM infiltrations to the normal bladder tissue and the tumor were estimated by the use of the photonic microscope system, since both drugs were known to emit characteristic fluorescence. It was found that infiltration of THP to the tumor tissue was more prominent in amounts and deeper than that of ADM, while smaller amounts of THP infiltrated into the normal mucosa compared to ADM. The fact might explain the clinical finding that THP instilled intravesically in half a concentration of ADM showed the same effect on the tumor as ADM.Subsequently, tissue concentrations of THP and ADM were estimated by high perfomance liquid chromatography. Either THP or ADM was instilled intravesically for 30 minutes to 6 rats with bladder tumor. Similarly, either THP or ADM was instilled in 5rats without the tumors. Contrary to the result of the photonic microscope system, the tissue concentration of THP was not different from that of ADM not only in the tumor tissues but also in the normal bladder ones. Furthermore, the tissue concentration of both drugs in the normal bladder was higher than that in the bladder tumor. The causes were considered as follows: since the amount of infiltrated drugs was very small in the face of the large amount of removed tissues, it was difficult to find the difference between both drugs. The weight of tumor tissues was so much larger than the normal bladder tissues that the tissue concentration of both drugs in the bladder tumor was low compared to that in the normal bladder.