학술논문
Robust Hepatitis C Virus Infection in vitro
Document Type
research-article
Author
Source
Proceedings of the National Academy of Sciences of the United States of America, 2005 Jun . 102(26), 9294-9299.
Subject
Language
English
ISSN
00278424
Abstract
The absence of a robust cell culture model of hepatitis C virus (HCV) infection has severely limited analysis of the HCV life cycle and the development of effective antivirals and vaccines. Here we report the establishment of a simple yet robust HCV cell culture infection system based on the HCV JFH-1 molecular clone and Huh-7-derived cell lines that allows the production of virus that can be efficiently propagated in tissue culture. This system provides a powerful tool for the analysis of host-virus interactions that should facilitate the discovery of antiviral drugs and vaccines for this important human pathogen.