학술논문
Release of Clathrin from Coated Vesicles Dependent upon a Nucleoside Triphosphate and a Cytosol Fraction
Document Type
research-article
Source
The Journal of Cell Biology, 1982 Apr 01. 93(1), 230-236.
Subject
Language
English
ISSN
00219525
Abstract
Calf-brain coated vesicles were incubated with ATP and a cytosol fraction. As much as 90% of the clathrin was selectively released within 10 min at 37°C without detectable proteolysis. This uncoating process required the presence of both ATP and cytosol. Empty cages of clathrin could also be dissociated in a similar manner. A nonhydrolyzable analogue, 5′-adenylylimidodiphosphate (AMP-PNP), would not substitute for ATP. Clathrin was dissociated from coats in a form unable to reassemble into cages under standard conditions. These reactions may reflect a segment of a clathrin-coated vesicle cycle in which coats are removed from vesicles after budding.