학술논문
Establishment of Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts From Fresh Tissue
Document Type
research-article
Author
Jesús Frutos Díaz-Alejo, author; Simon April-Monn, author; Marina Cihova, author; Verona Buocikova, author; Jorge Villalón López, author; Maria Urbanova, author; Carmen G. Lechuga, author; Miroslav Tomas, author; Peter Dubovan, author; Bárbara Luna Sánchez, author; Sonia Camaño Páez, author; Alfonso Sanjuanbenito, author; Eduardo Lobo, author; Estefanía Romio de la Heras, author; Carmen Guerra, author; Carolina de la Pinta, author; Emma Barreto Melian, author; Mercedes Rodríguez Garrote, author; Alfredo Carrato, author; Laura Ruiz-Cañas, author; Bruno Sainz, Jr, author; Ana Torres, author; Bozena Smolkova, author; Julie Earl, author
Source
Journal of Visualized Experiments. (195)
Subject
Language
English
ISSN
1940-087X
Abstract
Tumor organoids are three-dimensional (3D) ex vivo tumor models that recapitulate the biological key features of the original primary tumor tissues. Patient-derived tumor organoids have been used in translational cancer research and can be applied to assess treatment sensitivity and resistance, cell-cell interactions, and tumor cell interactions with the tumor microenvironment. Tumor organoids are complex culture systems that require advanced cell culture techniques and culture media with specific growth factor cocktails and a biological basement membrane that mimics the extracellular environment. The ability to establish primary tumor cultures highly depends on the tissue of origin, the cellularity, and the clinical features of the tumor, such as the tumor grade. Furthermore, tissue sample collection, material quality and quantity, as well as correct biobanking and storage are crucial elements of this procedure. The technical capabilities of the laboratory are also crucial factors to consider. Here, we report a validated SOP/protocol that is technically and economically feasible for the culture of ex vivo tumor organoids from fresh tissue samples of pancreatic adenocarcinoma origin, either from fresh primary resected patient donor tissue or patient-derived xenografts (PDX). The technique described herein can be performed in laboratories with basic tissue culture and mouse facilities and is tailored for wide application in the translational oncology field.