부산대학교 도서관

Keywords SARS-CoV-2; COVID-19; CD8+ T cells; vaccine; epitopes; variants; sarbecovirus; protection Highlights * Structure-based network analysis identifies mutation-constrained residues in SARS-CoV-2 * Highly networked residues are conserved across SARS-CoV-2 variants and sarbecoviruses * HLA stabilization defines highly networked epitopes with limited variation in VOCs * Highly networked epitopes elicit CD8.sup.+ T cell reactivity in recovered individuals Summary The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants that escape convalescent and vaccine-induced antibody responses has renewed focus on the development of broadly protective T-cell-based vaccines. Here, we apply structure-based network analysis and assessments of HLA class I peptide stability to define mutationally constrained CD8.sup.+ T cell epitopes across the SARS-CoV-2 proteome. Highly networked residues are conserved temporally among circulating variants and sarbecoviruses and disproportionately impair spike pseudotyped lentivirus infectivity when mutated. Evaluation of HLA class I stabilizing activity for 18 globally prevalent alleles identifies CD8.sup.+ T cell epitopes within highly networked regions with limited mutational frequencies in circulating SARS-CoV-2 variants and deep-sequenced primary isolates. Moreover, these epitopes elicit demonstrable CD8.sup.+ T cell reactivity in convalescent individuals but reduced recognition in recipients of mRNA-based vaccines. These data thereby elucidate key mutationally constrained regions and immunogenic epitopes in the SARS-CoV-2 proteome for a global T-cell-based vaccine against emerging variants and SARS-like coronaviruses.