학술논문
Application of the modular approach to an in-house validation study of real-time PCR methods for the detection and serogroup determination of verocytotoxigenci Escherichia coli
Document Type
Report
Author
Source
Applied and Environmental Microbiology. Oct, 2011, Vol. 77 Issue 19, p6954, 10 p.
Subject
Language
English
ISSN
0099-2240
Abstract
A 'modular approach' to the in-house validation of PCR methods proposed by the European Reference Laboratory for verocytotoxigenic Escherichia coli (VTEC) (EURL-VTEC) was applied to monitor E. coli for the microbiological criteria for food as an indicator of hygienic conditions recommended by European Commission regulation 2073/005. The study demonstrated the application of modular approach for the validation of PCR methods in food microbiology, using single-target plasmids as positive controls and food matrices.