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Byline: Geovani Alves dos Santos, Graduate Program on Physical Education, Federal University of San Francisco Valley, UNIVASF, PE, Petrolina, Brazil;, College UNINASSAU Petrolina, PE, Petrolina, Brazil;; Eduardo Seiji Numata-Filho, Graduate Program on Physical Education, Federal University of San Francisco Valley, UNIVASF, PE, Petrolina, Brazil;, College UNINASSAU Petrolina, PE, Petrolina, Brazil;, Tutorial Education Program, PET-Physical Education, UNIVASF, PE, Petrolina, Brazil; and; Thiago dos Santos Rosa, Graduate Program on Physical Education, Catholic University of Brazil, DF, BrasÈ¡lia, Brazil; Rodrigo Vanerson Passos Neves, Graduate Program on Physical Education, Catholic University of Brazil, DF, BrasÈ¡lia, Brazil; Herbert Gustavo SimÈ´es, Graduate Program on Physical Education, Catholic University of Brazil, DF, BrasÈ¡lia, Brazil; SȨrgio Rodrigues Moreira, Graduate Program on Physical Education, Federal University of San Francisco Valley, UNIVASF, PE, Petrolina, Brazil;, Tutorial Education Program, PET-Physical Education, UNIVASF, PE, Petrolina, Brazil; and Abstract Santos, GAd, Numata-Filho, ES, Rosa, TdS, Passos Neves, RV, SimÈ´es, HG, and Moreira, SR. Anaerobic threshold determination in cycle ergometer from rating of perceived exertion. J Strength Cond Res 36(5): 1277-1281, 2022--The objective of the study was to investigate the validity of the rating of perceived exertion threshold (TRPE) for anaerobic threshold (AT) estimation during an incremental test (IT) on a cycle ergometer. Nineteen physically active and healthy men performed an IT on a cycle ergometer. The intensity corresponding to the AT was considered as the point immediately before the abrupt increase in blood lactate concentration ([La]) in the IT. TRPE was determined in the last intensity corresponding to perceptual response previous the 15 arbitrary units (AU) in the 15-point RPE scale. The heart rate (HR), [La], and RPE were obtained at the end of each stage of the IT. There was no significant difference between workload obtained from the AT (150 [120-150] watts) and TRPE (150 [120-150] watts) (p > 0.05). In addition, there were no differences between HR (AT, 139.5 Ø 12.7 bømin-1 vs. TRPE, 141.9 Ø 14.6 bømin-1, p > 0.05) and [La] (AT, 3.1 [2.8-3.2] mM vs. TRPE, 3.1 [2.9-3.7] mM, p > 0.05). There was a strong correlation between the intensities in AT and TRPE during the IT (r = 0.88). A substantial reliability (intraclass correlation coefficient = 0.84 [0.64-0.94 confidence interval 95%]) was evidenced, as well as a substantial agreement between the AT and TRPE intensities (Pc = 0.84), as confirmed by the Bland-Altman plot (-4.7 [-34.2/24.7]). TRPE was a predictor of the AT (ð = 1.146, R = 0.770, SEE = 15.070, p < 0.01). It can be concluded that TRPE is valid for AT estimation during an IT on a cycle ergometer.