학술논문
Mucosal adhesion properties of the probiotic Lactobacillus rhamnosus GG SpaCBA and SpaFED pilin subunits
Document Type
Report
Author
Source
Applied and Environmental Microbiology. April 2010, Vol. 76 Issue 7, p2049, 9 p.
Subject
Language
English
ISSN
0099-2240
Abstract
The pilin genes (spaA, spaB, spaD, spaE, and spaF) Escherichia coli was cloned and the recombinant proteins were produced and purified to assess the adherence of Lactobacillus rhamnosus GG spaCBA and spaFED pilin proteins to human intestinal mucus. The results suggested that the two pilin subunits (spaB and spaC) in the spaCBA pilus fiber play a critical role in binding to intestinal mucus while for the uncharacterized and putative spaFED pilus fiber only a single pilin subunit (spaF) is potentially responsible for adhesion to mucus.