학술논문
Use of Hupresin To Capture Red Blood Cell Acetylcholinesterase for Detection of Soman Exposure
Document Type
Report
Author
Source
Analytical Chemistry. Jan 2, 2018, Vol. 90 Issue 1, p974, 6 p.
Subject
Language
English
ISSN
0003-2700
Abstract
The article provides an alternative RBC acetylcholinesterase (AChE) enrichment strategy, by binding RBC AChE to Hupresin affinity gel. Hemoglobin and other proteins are washed off with 3 M NaCl, while retaining AChE bound to Hupresin. It is concluded that Hupresin can be used to enrich soman-inhibited AChE solubilized from 8 mL of frozen human erythrocytes, yielding a quantity sufficient for detecting soman exposure.