부산대학교 도서관

The article describes the investigation of the interaction of single stranded DNA labeled with either fluorescein (FAM) or tetramethylrhodamine (TAMRA) with a metal surface, using spermine induced aggregated silver nanoparticles as the surface enhanced Raman scattering (SERS) substrate, by analyzing the labels separately and in mixtures. It is found that when the two dyes are premixed prior to the addition of nanoparticles, a strong masking effect over FAM is exerted by TAMRA due to a stronger affinity for the metal surface; moreover, when parameters such as order of analyte addition, analysis time, and analyte concentration are examined, the masking effect of TAMRA is still observed but the extent changes depending on the experimental parameters. It is shown that that the order of addition of the analytes is important and that specific dye related interactions occur, greatly affecting the observed SERS spectra.