부산대학교 도서관

A new liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method has been developed and validated for alfuzosin quantification in human plasma using amlodipine as an internal standard (IS). The analyte and IS were extracted using a mixture of diethyl ether-dichloroethane (70 : 30, v/v). The samples were separated on a Waters XBridge HILIC column, where a tandem mass spectrometer with a turbo ion spray interface was used for analytes detection. The chromatographic run time of the method was 4.0 min. The method was linear over the concentration range of 0.20-30 ng/mL for alfuzosin. On evaluating the selectivity of the method, no interference was observed for the two MRM transitions of alfuzosin and MRM transition for amlodipine at their respective retention times. The mean matrix effect for alfuzosin was excellent at 99.9%. The method was carefully tested for linearity, system suitability, precision and accuracy. Intra- and inter-day accuracy and precision were evaluated at three quality control levels (LQC, MQC and HQC). Stability of alfuzosin was evaluated under diverse storage conditions. The percentage accuracy for 1 : 2 diluted samples was 93.2% of their nominal concentration with the coefficient of variation of 5.6%. Hence, a highly precise, accurate, rapid, selective and sensitive LC-ESI-MS/MS method has been developed and validated for the quantification of alfuzosin in human plasma using amlodipine as the internal standard.
Author(s): Moustapha E. Moustapha [sup.1] [sup.2], Rania M. El Gamal [sup.3] [sup.4], Mehnaz Kamal [sup.4] Author Affiliations: (1) grid.449553.a, 0000 0004 0441 5588, Department of Chemistry, College of Science and [...]