부산대학교 도서관

To purchase or authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1745-4522.2008.00124.x Byline: TERESA LOPES DA SILVA (1,3), ELINA C. BERNARDO (1), BEATRIZ NOBRE (2), RUI L. MENDES (2), ALBERTO REIS (1) Abstract: ABSTRACT SFE of the oils of two varieties of grape seeds (Victoria and Red Globe) was performed in a flow apparatus at 40C and 250 bar. The effect of the solvent on the extraction yield was studied using CO.sub.2 and CO.sub.2 modified with ethanol, and the yield of extraction was compared with conventional extraction methods (Bligh and Dyer and Soxhlet using hexane). SC CO.sub.2 + ethanol extracted higher lipid amounts from both varieties, compared with SC CO.sub.2 extraction. The comparison between SFE and conventional extraction methods showed that the highest extraction yield was obtained with SC CO.sub.2 + ethanol (10%, w/w) (14.7 and 11.8% for Red Globe and Victoria grape seeds, respectively), while pure SC CO.sub.2 afforded the lowest lipid yields. The fatty acid compositions of the oils obtained by SFE were analyzed. Fractions containing higher proportions of PUFAs were obtained at lower solvent/dry grape seed ratios, while fractions richer in SFAs and MUFAs were obtained at higher solvent/dry grape seed ratios. PRACTICAL APPLICATIONS SFE could fractionate grape seed oil and afford various grades of oil containing different proportions of fatty acids. The highest grape seed lipid yield obtained was with SC CO.sub.2 + ethanol, making this solvent a promising and powerful one for grape seed oil extraction, showing a great advantage compared with conventional extraction methods. Author Affiliation: (1)Unidade de Bioengenharia e BioprocessosDepartamento de Biotecnologia (2)Unidade de BiomassaDepartamento de Energias RenovaveisInstituto Nacional de EngenhariaTecnologia e InovacaoEstrada do Paco do Lumiar, 22, 1649-038 Lisbon, Portugal Article History: Submitted for Publication January 24, 2008 Revised Received and Accepted March 11, 2008 Article note: (3) TEL: +351-21-0924600; FAX: +351-21-7163636; EMAIL: teresa.lopesilva@ineti.pt