학술논문
PCR-based diagnosis for detection of Leishmania in skin and blood of rodents from an endemic area of cutaneous and visceral leishmaniasis in Brazil
Document Type
Report
Author
Source
Veterinary Parasitology. May 15, 2005, Vol. 129 Issue 3-4, p219, 9 p.
Subject
Language
English
ISSN
0304-4017
Abstract
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.vetpar.2005.01.005 Byline: Fernanda S. Oliveira (a), Claude Pirmez (b), Marize Q. Pires (a), Reginaldo P. Brazil (c), Raquel S. Pacheco (a) Keywords: Leishmania; PCR; Hybridization; Rodents; Reservoirs; Molecular epidemiology Abstract: The technique of polymerase chain reaction (PCR) associated to hybridization was used to screen 123 samples collected from wild and synanthropic rodents captured in a cutaneous and visceral leishmaniasis endemic area in the State of Minas Gerais, Brazil. The detection of Leishmania spp in naturally infected rodents is of fundamental importance for incriminating them as possible reservoir hosts of the diseases in Minas Gerais. A total of 62 specimens belonging to wild (Thrichomys apereoides, Oryzomys subflavus, Galea spixii, Bolomys lasiurus and Wiedomys pyrrhorhinos) and synanthropic (R. rattus) rodent species were captured in different ecotopes. Blood and skin samples were submitted for PCR analyses followed by molecular hybridization with specific probes for the three Leishmania-species complexes. Fifteen samples were found positive after PCR-hybridization and identified as follows: nine belonging to the L. mexicana complex, three to the L. braziliensis complex and three to the L. donovani complex. Positive PCR results were found in 11 out of the 61 (18%) blood samples and in four out of the 62 (6.4%) skin fragments screened. R. rattus and T. apereoides were the most abundant species in the area also presenting high prevalence of natural infection. The presence of parasite DNA belonging to L. braziliensis, L. mexicana and L. donovani complexes was confirmed in several individuals of a rodent species, R. rattus. This work is the first report of the detection of L. (L.) chagasi in a naturally infected T. apereoides. The utility of filter paper as a substrate for PCR analyses and the efficacy of the procedure associated to the hybridization is emphasized. Author Affiliation: (a) Laboratorio de Sistematica Bioquimica, Departamento de Bioquimica e Biologia Molecular (DBBM), Instituto Oswaldo Cruz (IOC), Fundacao Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brasil (b) Laboratorio de Imunopatologia, DBBM, IOC/FIOCRUZ, Rio de Janeiro, Brasil (c) Laboratorio de Bioquimica e Fisiologia de Insetos, DBBM, IOC/FIOCRUZ, Rio de Janeiro, Brasil Article History: Accepted 11 January 2005