학술논문
Thionein can serve as a reducing agent for the methionine sulfoxide reductases
Document Type
Author abstract
Author
Source
Proceedings of the National Academy of Sciences of the United States. June 6, 2006, Vol. 103 Issue 23, p8656, 6 p.
Subject
Language
English
ISSN
0027-8424
Abstract
It has been generally accepted, primarily from studies on methionine sulfoxide reductase (Msr) A, that the biological reducing agent for the members of the Msr family is reduced thioredoxin (Trx), although high levels of DTT can be used as the reductant in vitro. Preliminary experiments using both human recombinant MsrB2 (hMsrB2) and MsrB3 (hMsrB3) showed that although DTT can function in vitro as the reducing agent, Trx works very poorly, prompting a more careful comparison of the ability of DTT and Trx to function as reducing agents with the various members of the Msr family. Escherichia coil MsrA and MsrB and bovine MsrA efficiently use either Trx or DTT as reducing agents. In contrast, hMsrB2 and hMsrB3 show metallothioneins | reducing systems