학술논문
Production of the antifungal biopesticide physcion through the combination of microbial fermentation and chemical post-treatment
Research
Research
Document Type
Report
Author
Source
Bioresources and Bioprocessing. December 2023, Vol. 10 Issue 1
Subject
Language
English
Abstract
Author(s): Zheng Zhuang [sup.1] , Xueqing Zhong [sup.1] , Qinghua Li [sup.1] , Tian Liu [sup.4] , Qing Yang [sup.2] , Guo-Qiang Lin [sup.1] , Qing-Li He [sup.1] , Qunfei [...]
Physcion is an anthraquinone compound observed dominantly in medicinal herbs. This anthraquinone possesses a variety of pharmaceutically important activities and has been developed to be a widely used antifungal biopesticide. Herein, we report on the effective preparation of 3R-torosachrysone (4), a tetrahydroanthracene precursor of physcion, in Aspergillus oryzae NSAR1 by heterologous expression of related genes mined from the phlegmacins-producing ascomycete Talaromyces sp. F08Z-0631. Conditions for converting 4 into physcion were studied and optimized, leading to the development of a concise approach for extracting high-purity physcion from the alkali-treated fermentation broth of the 4-producing A. oryzae strain. Graphical Abstract
Physcion is an anthraquinone compound observed dominantly in medicinal herbs. This anthraquinone possesses a variety of pharmaceutically important activities and has been developed to be a widely used antifungal biopesticide. Herein, we report on the effective preparation of 3R-torosachrysone (4), a tetrahydroanthracene precursor of physcion, in Aspergillus oryzae NSAR1 by heterologous expression of related genes mined from the phlegmacins-producing ascomycete Talaromyces sp. F08Z-0631. Conditions for converting 4 into physcion were studied and optimized, leading to the development of a concise approach for extracting high-purity physcion from the alkali-treated fermentation broth of the 4-producing A. oryzae strain. Graphical Abstract