학술논문

Cross-amplification and characterization of microsatellite loci for three species of Theobroma (Sterculiaceae) from the Brazilian Amazon
Document Type
Report
Author abstract
Source
Genetic Resources and Crop Evolution. Dec, 2007, Vol. 54 Issue 8, p1653, 5 p.
Subject
Satellite DNA
Language
English
ISSN
0925-9864
Abstract
This study reports on the cross-species amplification of 23 microsatellite markers previously developed for Theobroma cacao L. (Sterculiaceae), source of chocolate in three economically important Amazonian species of Theobroma (T. grandiflorum, T. subincanum, T. sylvestre). Thirteen of the 23 microsatellite loci tested were polymorphic across the three species at 2--13 alleles per locus. The observed heterozygosity per locus varied from 0.18 to 0.84 and expected heterozygosity ranged from 0.28 to 0.87. The high level of transferability and genetic information content of these microsatellite loci indicate their usefulness for population genetic, mating system and breeding studies of these economically important Amazonian fruit trees.