학술논문
CENPA promotes clear cell renal cell carcinoma progression and metastasis via Wnt/[beta]-catenin signaling pathway
centromere protein A
centromere protein A
Document Type
Report
Author
Source
Journal of Translational Medicine. October 9, 2021, Vol. 19 Issue 1
Subject
Language
English
ISSN
1479-5876
Abstract
Author(s): Qi Wang[sup.1] , Jiaju Xu[sup.1] , Zhiyong Xiong[sup.1] , Tianbo Xu[sup.1] , Jingchong Liu[sup.1] , Yuenan Liu[sup.1] , Jiaping Chen[sup.3] , Jian Shi[sup.1] , Yi Shou[sup.1] , Changjie Yue[sup.1] [...]
Clear cell renal cell carcinoma (ccRCC) is the most common malignant tumor of the kidney. New and reliable biomarkers are in urgent need for ccRCC diagnosis and prognosis. The CENP family is overexpressed in many types of cancers, but its functions in ccRCC have not been fully clarified. In this paper, we found that several CENP family members were highly expressed in ccRCC tissues. Also, CENPA expression level was related to clinicopathological grade and prognosis by weighted gene co-expression network analysis (WGCNA). CENPA served as a representative CENP family member as a ccRCC biomarker. Further in vitro experiments verified that overexpression of CENPA promoted ccRCC proliferation and metastasis by accelerating the cell cycle and activating the Wnt/[beta]-catenin signaling pathway. The elevated [beta]-catenin led by CENPA overexpression translocated to nucleus for downstream effect. Functional recovery experiment confirmed that Wnt/[beta]-catenin pathway was essential for ccRCC progression and metastasis. Developing selective drugs targeting CENPA may be a promising direction for cancer treatment. Keywords: Kidney renal cell carcinoma, Biomarker, Gene set enrichment analysis, Metastasis, CENPA, Targeted therapy
Clear cell renal cell carcinoma (ccRCC) is the most common malignant tumor of the kidney. New and reliable biomarkers are in urgent need for ccRCC diagnosis and prognosis. The CENP family is overexpressed in many types of cancers, but its functions in ccRCC have not been fully clarified. In this paper, we found that several CENP family members were highly expressed in ccRCC tissues. Also, CENPA expression level was related to clinicopathological grade and prognosis by weighted gene co-expression network analysis (WGCNA). CENPA served as a representative CENP family member as a ccRCC biomarker. Further in vitro experiments verified that overexpression of CENPA promoted ccRCC proliferation and metastasis by accelerating the cell cycle and activating the Wnt/[beta]-catenin signaling pathway. The elevated [beta]-catenin led by CENPA overexpression translocated to nucleus for downstream effect. Functional recovery experiment confirmed that Wnt/[beta]-catenin pathway was essential for ccRCC progression and metastasis. Developing selective drugs targeting CENPA may be a promising direction for cancer treatment. Keywords: Kidney renal cell carcinoma, Biomarker, Gene set enrichment analysis, Metastasis, CENPA, Targeted therapy