학술논문
Molecular characterization and antiviral analyses of porcine type III interferons
Document Type
Report
Source
Journal of Interferon & Cytokine Research. November 1, 2010, Vol. 30 Issue 11, p801, 7 p.
Subject
Language
English
ISSN
1079-9907
Abstract
INTERFERONS (IFNs) ARE CLASSIFIED INTO 3 families, namely types I, II, and III (Ank and others 2006; Takeuchi and Akira 2009). A single type II IFN, ie, IFN-γ, is primarily [...]
Type III interferons (IFNs) are a family of recently identified antiviral cytokines. One to 3 paralogs have been identified in several species; however, little information is available about type III IFNs in pigs. We have identified 2 porcine type III IFNs, Sus scrofa IFN-λ1 (SsIFN-λ1) and SsIFN-λ3, and determined their tissue expression profile and antiviral activities. Open reading frames of SsIFN-λ1 and SsIFN-λ3 are 576 and 588 bp, encoding 191 and 195 amino acid preproteins, respectively. In healthy pigs, SsIFN-λ3 was primarily expressed in mesenteric lymph nodes and intestine, whereas expression of SsIFN-λ1 was found in all tested tissues and was high in mesenteric lymph nodes, intestine, and liver. Porcine cells treated with the viral mimic, dsRNA, robustly increased SsIFN-λ3 expression, with epithelial cells generally displaying the greatest response. Conversely, dsRNA-induced mRNA expressions of SsIFN-λ1, SsIFN-α1, and SsIFN-β were relatively weaker and delayed compared with SsIFN-λ3. SsIFN-λ1 and SsIFN-λ3 peptides exerted similar but lower antiviral potency than SsIFN-α1 and SsIFN-β against a porcine arterivirus and an adenovirus. These findings indicate that pigs have 2 type III IFN paralogs, which have antiviral activity and may serve as targets for modulation of the porcine host-- pathogen interaction.
Type III interferons (IFNs) are a family of recently identified antiviral cytokines. One to 3 paralogs have been identified in several species; however, little information is available about type III IFNs in pigs. We have identified 2 porcine type III IFNs, Sus scrofa IFN-λ1 (SsIFN-λ1) and SsIFN-λ3, and determined their tissue expression profile and antiviral activities. Open reading frames of SsIFN-λ1 and SsIFN-λ3 are 576 and 588 bp, encoding 191 and 195 amino acid preproteins, respectively. In healthy pigs, SsIFN-λ3 was primarily expressed in mesenteric lymph nodes and intestine, whereas expression of SsIFN-λ1 was found in all tested tissues and was high in mesenteric lymph nodes, intestine, and liver. Porcine cells treated with the viral mimic, dsRNA, robustly increased SsIFN-λ3 expression, with epithelial cells generally displaying the greatest response. Conversely, dsRNA-induced mRNA expressions of SsIFN-λ1, SsIFN-α1, and SsIFN-β were relatively weaker and delayed compared with SsIFN-λ3. SsIFN-λ1 and SsIFN-λ3 peptides exerted similar but lower antiviral potency than SsIFN-α1 and SsIFN-β against a porcine arterivirus and an adenovirus. These findings indicate that pigs have 2 type III IFN paralogs, which have antiviral activity and may serve as targets for modulation of the porcine host-- pathogen interaction.