학술논문
Assessing Cellular Uptake of Exogenous Coenzyme Q[sub.10] into Human Skin Cells by X-ray Fluorescence Imaging
Document Type
Report
Author
Source
Antioxidants. August 2022, Vol. 11 Issue 8
Subject
Language
English
ISSN
2076-3921
Abstract
Author(s): Theresa Staufer (corresponding author) [1,*]; Mirja L. Schulze [2]; Oliver Schmutzler [1]; Christian Körnig [1]; Vivienne Welge [2]; Thorsten Burkhardt [2]; Jens-Peter Vietzke [2]; Alexandra Vogelsang [2]; Julia M. [...]
X-ray fluorescence (XRF) imaging is a highly sensitive non-invasive imaging method for detection of small element quantities in objects, from human-sized scales down to single-cell organelles, using various X-ray beam sizes. Our aim was to investigate the cellular uptake and distribution of Q[sub.10] , a highly conserved coenzyme with antioxidant and bioenergetic properties. Q[sub.10] was labeled with iodine (I[sub.2] -Q[sub.10] ) and individual primary human skin cells were scanned with nano-focused beams. Distribution of I[sub.2] -Q[sub.10] molecules taken up inside the screened individual skin cells was measured, with a clear correlation between individual Q[sub.10] uptake and cell size. Experiments revealed that labeling Q[sub.10] with iodine causes no artificial side effects as a result of the labeling procedure itself, and thus is a perfect means of investigating bioavailability and distribution of Q[sub.10] in cells. In summary, individual cellular Q[sub.10] uptake was demonstrated by XRF, opening the path towards Q[sub.10] multi-scale tracking for biodistribution studies.
X-ray fluorescence (XRF) imaging is a highly sensitive non-invasive imaging method for detection of small element quantities in objects, from human-sized scales down to single-cell organelles, using various X-ray beam sizes. Our aim was to investigate the cellular uptake and distribution of Q[sub.10] , a highly conserved coenzyme with antioxidant and bioenergetic properties. Q[sub.10] was labeled with iodine (I[sub.2] -Q[sub.10] ) and individual primary human skin cells were scanned with nano-focused beams. Distribution of I[sub.2] -Q[sub.10] molecules taken up inside the screened individual skin cells was measured, with a clear correlation between individual Q[sub.10] uptake and cell size. Experiments revealed that labeling Q[sub.10] with iodine causes no artificial side effects as a result of the labeling procedure itself, and thus is a perfect means of investigating bioavailability and distribution of Q[sub.10] in cells. In summary, individual cellular Q[sub.10] uptake was demonstrated by XRF, opening the path towards Q[sub.10] multi-scale tracking for biodistribution studies.