부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.procbio.2008.06.012 Byline: Ines Garbayo (a), Maria Cuaresma (a), Carlos Vilchez (a), Jose M. Vega (b) Keywords: Extremophiles; Chlamydomonas acidophila; Environmental stress; Carotenoids; Lutein; [beta]-Carotene Abstract: Chlamydomonas acidophila growing autotrophically with continuous PAR light (160[mu]Em.sup.-2 s.sup.-1) and 30[degrees]C may accumulate carotenoids which increase in response to abiotic stress, like high light intensity, UV-A radiation and temperature fluctuation. At PAR light of 240[mu]Em.sup.-2 s.sup.-1 the alga contains, after 20 days of growth, 57.5[+ or -]1.6mgl.sup.-1 of total carotenoids, which does not significantly change by an irradiance of 1000[mu]Em.sup.-2 s.sup.-1. Lutein (20[+ or -]0.5mgl.sup.-1) and [beta]-carotene (8.3[+ or -]0.2mgl.sup.-1) production were particularly high in C. acidophila, while zeaxanthin (0.2[+ or -]0.1mgl.sup.-1) was low. Enhanced production of lutein and [beta]-carotene were observed in the alga grown under standard conditions, but either supplemented with moderate UV-A radiation (10[mu]Em.sup.-2 s.sup.-1), or heated at 40[degrees]C. The presence of Fe.sup.3+ in the culture medium, in a range between 5 and 35mM, significantly decreased the C. acidophila viability and the intracellular carotenoids content. However Cu.sup.2+, at 1-5mM, appears to increase the synthesis of [beta]-carotene. The alga can grow under mixotrophic conditions, with glucose, 10mM, as carbon source, but any of such conditions improved the intracellular content of carotenoids. Author Affiliation: (a) Departamento de Quimica y Ciencia de los Materiales "Profesor J.C. Vilchez Martin", Facultad de Ciencias Experimentales, Universidad de Huelva, Huelva 21017, Spain (b) Departamento de Bioquimica Vegetal y Biologia Molecular, Facultad de Quimica, Universidad de Sevilla, c/o Prof. Garcia Gonzalez, No. 1, Sevilla 41071, Spain Article History: Received 24 June 2008; Accepted 24 June 2008