학술논문
Three-Dimensional Analysis of Round Window Membrane in the Chinchilla Model with Acute Otitis Media Induced with Streptococcus Pneumoniae 7F
Original Investigation
Original Investigation
Document Type
Report
Author
Source
Turkish Archives of Otorhinolaryngology. March 2021, Vol. 59 Issue 1, p43, 6 p.
Subject
Language
English
ISSN
2149-3987
Abstract
Introduction Acute Otitis media (AOM), which defines the inflammation of the tympanic cavity, the Eustachian tube, the mastoid process and the mucoperiosteum surrounding the mastoid air cells, is recognized as [...]
Objective: The purpose of this study was to investigate the morphological changes of round window membrane (RWM) in chinchillas with Streptococcus pneumoniae (S. pneumoniae) serotype 7F induced acute otitis media (AOM) by two dimensional (2D) and three dimensional (3D) measurements. Methods: Temporal bone specimens taken from 12 chinchillas were divided into two groups. The control group consisted of healthy animals that were injected with intrabullar saline. The subjects in the experimental group were induced with AOM by intrabullar injection of S. pneumoniae 7F. The 2D and 3D measurements of RWM were compared between the groups. Results: Dramatic changes were noted in the RWM of the experimental group compared to the control group. The thickness [mean [+ or -] standard deviation (SD)] of the RWM was significantly (p Conclusion: The results of our study, which indicated significant change in RWM in both 2D and 3D measurements, may shed light on the relationship between AOM and inner ear diseases. Based on our results, we recommend evaluating 3D analyses of RWM, which provide useful data, to better understand the changes in the membrane. Keywords: Acute otitis media, pathology, cochlear round window, Streptococcus pneumoniae, cellular morphology
Objective: The purpose of this study was to investigate the morphological changes of round window membrane (RWM) in chinchillas with Streptococcus pneumoniae (S. pneumoniae) serotype 7F induced acute otitis media (AOM) by two dimensional (2D) and three dimensional (3D) measurements. Methods: Temporal bone specimens taken from 12 chinchillas were divided into two groups. The control group consisted of healthy animals that were injected with intrabullar saline. The subjects in the experimental group were induced with AOM by intrabullar injection of S. pneumoniae 7F. The 2D and 3D measurements of RWM were compared between the groups. Results: Dramatic changes were noted in the RWM of the experimental group compared to the control group. The thickness [mean [+ or -] standard deviation (SD)] of the RWM was significantly (p Conclusion: The results of our study, which indicated significant change in RWM in both 2D and 3D measurements, may shed light on the relationship between AOM and inner ear diseases. Based on our results, we recommend evaluating 3D analyses of RWM, which provide useful data, to better understand the changes in the membrane. Keywords: Acute otitis media, pathology, cochlear round window, Streptococcus pneumoniae, cellular morphology