부산대학교 도서관

Background Acute respiratory distress syndrome (ARDS) is responsible for 400,000 deaths annually worldwide. Few improvements have been made despite five decades of research, partially because ARDS is a highly heterogeneous syndrome including various types of aetiologies. Lower airway microbiota is involved in chronic inflammatory diseases and recent data suggest that it could also play a role in ARDS. Nevertheless, whether the lower airway microbiota composition varies between the aetiologies of ARDS remain unknown. The aim of this study is to compare lower airway microbiota composition between ARDS aetiologies, i.e. pulmonary ARDS due to influenza, SARS-CoV-2 or bacterial infection. Methods Consecutive ARDS patients according to Berlin's classification requiring invasive ventilation with PCR-confirmed influenza or SARS-CoV-2 infections and bacterial infections (> 105 CFU/mL on endotracheal aspirate) were included. Endotracheal aspirate was collected at admission, V3-V4 and ITS2 regions amplified by PCR, deep-sequencing performed on MiSeq sequencer (Illumina[R]) and data analysed using DADA2 pipeline. Results Fifty-three patients were included, 24 COVID-19, 18 influenza, and 11 bacterial CAP-related ARDS. The lower airway bacteriobiota and mycobiota compositions ([beta]-diversity) were dissimilar between the three groups (p = 0.05 and p = 0.01, respectively). The bacterial [alpha]-diversity was significantly lower in the bacterial CAP-related ARDS group compared to the COVID-19 ARDS group (p = 0.04). In contrast, influenza-related ARDS patients had higher lung mycobiota [alpha]-diversity than the COVID-19-related ARDS (p = 0 < 01). Conclusion Composition of lower airway microbiota (both microbiota and mycobiota) differs between influenza, COVID-19 and bacterial CAP-related ARDS. Future studies investigating the role of lung microbiota in ARDS pathophysiology should take aetiology into account. Keywords: Acute respiratory distress syndrome, Mycobiota, Microbiota
Author(s): Sébastien Imbert[sup.1,2] , Mathilde Revers[sup.1,2] , Raphaël Enaud[sup.2,3] , Arthur Orieux[sup.4] , Adrian Camino[sup.2] , Alexandre Massri[sup.5] , Laurent Villeneuve[sup.6] , Cédric Carrié[sup.7] , Laurent Petit[sup.7] , Alexandre Boyer[sup.2,4] [...]