학술논문
Rupture of the hydrogen bond linking two Omega-loops induces the molten globule state at neutral pH in cytochrome c
Document Type
Academic Journal
Author
Source
Biochemistry. June 24, 2003, Vol. 42 Issue 24, p7604
Subject
Language
English
ISSN
0006-2960
Abstract
Research has been conducted on cytochrome c His26Tyr and His33Tyr mutants. The authors have investigated the structural and functional properties of these mutants under physiological-like conditions in the effort to understand the role of His26-Glu44 hydrogen bond in native protein's rigid tertiary conformation stabilization, and to determine whether the lack of this hydrogen bond will force the protein to assume the molten globule-like structure at neutral pH.