학술논문
The emergence of blaCTX-M-15-carrying Escherichia coli of ST131 and new sequence types in Western China
Document Type
Report
Author
Source
Annals of Clinical Microbiology and Antimicrobials. November 27, 2013, Vol. 12
Subject
Language
English
ISSN
1476-0711
Abstract
Author(s): Lei Zhang[sup.1,2] , Xiaoju Lü[sup.1,2] and Zhiyong Zong[sup.1,2] Findings Escherichia coli producing extended-spectrum [beta]-lactamases (ESBLs) is a challenge for clinical treatment and infection control. bla [sub.CTX-M-15] has emerged as [...]
Background bla.sub.CTX-M-15, the most widely distributed gene encoding extended-spectrum [beta]-lactamases globally, was not common in China. This study was performed to characterize bla.sub.CTX-M-15-carrying Escherichia coli in western China. Findings Out of 144 Escherichia coli isolates from 20 hospitals in western China, 8 were found carrying bla.sub.CTX-M-15. bla.sub.CTX-M-15 was carried by isolates of ST131and 5 new STs (ST3342, ST3513, ST3516, ST3517 and ST3518). The 5 new STs shared 5 identical alleles out of 7 but only had up to 2 alleles identical to ST131. bla.sub.CTX-M-15 was located on plasmids of IncI1 (ST16) or IncFII-related group (four replicon types). The co-transfer of a few antimicrobial resistance genes including qnrA, qnrB, qnrS, qepA, aac (6')-Ib-cr, aac (3)-II, tetA, bla.sub.TEM and bla.sub.OXA-1 with bla.sub.CTX-M-15 were examined but only bla.sub.TEM-1 was found co-transferring with bla.sub.CTX-M-15. Conclusions Five new STs of E. coli and three new types of IncFII-related plasmids carrying bla.sub.CTX-M-15 were identified. This study together with several reports suggested that bla.sub.CTX-M-15 has emerged in China and the interruption of both vertical and horizontal transmission of bla.sub.CTX-M-15 is required to hurdle its further spread. Keywords: Escherichia coli, Plasmids, MLST, Beta-lactamases, Antimicrobial resistance
Background bla.sub.CTX-M-15, the most widely distributed gene encoding extended-spectrum [beta]-lactamases globally, was not common in China. This study was performed to characterize bla.sub.CTX-M-15-carrying Escherichia coli in western China. Findings Out of 144 Escherichia coli isolates from 20 hospitals in western China, 8 were found carrying bla.sub.CTX-M-15. bla.sub.CTX-M-15 was carried by isolates of ST131and 5 new STs (ST3342, ST3513, ST3516, ST3517 and ST3518). The 5 new STs shared 5 identical alleles out of 7 but only had up to 2 alleles identical to ST131. bla.sub.CTX-M-15 was located on plasmids of IncI1 (ST16) or IncFII-related group (four replicon types). The co-transfer of a few antimicrobial resistance genes including qnrA, qnrB, qnrS, qepA, aac (6')-Ib-cr, aac (3)-II, tetA, bla.sub.TEM and bla.sub.OXA-1 with bla.sub.CTX-M-15 were examined but only bla.sub.TEM-1 was found co-transferring with bla.sub.CTX-M-15. Conclusions Five new STs of E. coli and three new types of IncFII-related plasmids carrying bla.sub.CTX-M-15 were identified. This study together with several reports suggested that bla.sub.CTX-M-15 has emerged in China and the interruption of both vertical and horizontal transmission of bla.sub.CTX-M-15 is required to hurdle its further spread. Keywords: Escherichia coli, Plasmids, MLST, Beta-lactamases, Antimicrobial resistance