부산대학교 도서관

On a large plasmid of Acinetobacter johnsonii strain XBB1 from hospital sewage, bla.sub.PER-1 and ISCR1 were found in a complex Tn402-like integron carrying an arr3-aacA4 cassette array. The integron was truncated by the same 439-bp miniature inverted-repeat transposable element (MITE) at both ends. bla.sub.PER-1 and its complex surroundings might have been mobilized by the MITEst into an orf of unknown function, evidenced by the presence of the characteristic 5-bp direct target repeats. The same 439-bp MITEs have also been found flanking class 1 integrons carrying metallo-[beta]-lactamases genes bla.sub.IMP-1, bla.sub.IMP-5 and bla.sub.VIM-2 before but without ISCR1. Although the cassette arrays are different, integrons have always been truncated by the 439-bp MITEs at the exact same locations. The results suggested that MITEs might be able to mobilize class 1 integrons via transposition or homologous recombination and therefore represent a possible common mechanism for mobilizing antimicrobial resistance determinants.
Author(s): Zhiyong Zong 1,2,* Introduction bla [sub.PER-1] encodes the extended-spectrum [beta]-lactamase (ESBL) PER-1 conferring resistance to penicillins, cephalosporins and monobactams [1] and has been found in Aeromonas spp., Acinetobacter baumannii [...]