부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1078/143446103322166509 Byline: Catherine Klotz (a), Francoise Rulz (a), Nicole Garreau de Loubresse (a), Michel Wright (b), Pascale Dupuid-Williams (c), Janine Beisson (a) Keywords: MTOCs; Paramecium; ciliates; [gamma]-Tubulin; localization; cell cycle; microtubules Abstract: The characterization of the two Paramecium [gamma]-tubulin genes, [gamma]PT1 and [gamma]PT2, allowed us to raise Paramecium-specific antibodies, directed against their most divergent carboxy-terminal peptide and to analyze the localization and dynamics of [gamma]-tubulin throughout the cell cycle. As in other cell types, a large proportion of the protein was found to be cytosolic, but in contrast to the general situation, [gamma]-tubulin was found to be permanently associated to four types of sites: basal bodies, the micronuclear compartment - within which mitotic and meiotic spindles develop without membrane breakdown -, the pores of the contractile vacuoles and the cytoproct which are cortical microtubular organelles fulfilling excretory functions. In addition, a transient site of [gamma]-tubulin and microtubule assembly was observed at the site of nuclear exchange during conjugation. This complexity accounts for the nucleation of most of the numerous and diverse microtubule arrays present in Paramecium. The sites and mode of nucleation of the microtubule bundles formed in the macronuclear compartment during division remain unclear. These observations lead us to discuss the relationships between microtubules, [gamma]-tubulin and MTOCs. Author Affiliation: (a) Centre de Genetique Moleculaire du CNRS, 91190 Gif-sur-Yvette, France (b) Institut de Pharmacologie et Biologie Structurale du CNRS, 205 Route de Narbonne, 31400 Toulouse, France (c) Ecole Superieure de Physique et Chimie Industrielles, 10, rue Vauquelin, 75 005 Paris, France Article History: Received 31 October 2002; Accepted 4 February 2003