학술논문
Role of c-fos gene in vasoactive intestinal peptide promoted synthesis of pulmonary surfactant phospholipids
Document Type
Author abstract
Author
Source
Regulatory Peptides. May 3, 2007, Vol. 140 Issue 3, p117, 8 p.
Subject
Language
English
ISSN
0167-0115
Abstract
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.regpep.2006.11.027 Byline: Lian Li (a), Hua She (a), Shao-Jie Yue (b), Xiao-Qun Qin (a), Cha-Xiang Guan (a), Hui-Jun Liu (a), Zi-Qiang Luo (a) Keywords: Vasoactive intestinal peptide; Intracellular signal transduction pathway; Phosphatidylcholine; Protein kinase C; c-Fos protein Abstract: We previously reported that vasoactive intestinal peptide (VIP) promoted synthesis of phosphatidylcholine (PC) in alveolar type II (ATII) cells. But the intracellular mechanism for this effect was unknown. In this work, we investigated the intracellular signal transduction pathway for VIP promoted synthesis of PC, the major lipid component of pulmonary surfactant (PS), by using an antagonist of VIP receptors, inhibitor of protein kinase C (PKC) and antisense oligonucleotides (AS-ODN) for c-fos oncogene. Our results showed that: a [D-P-Cl-Phe(6)-Leu(17)]-VIP (10.sup.-6 mol/l), an antagonist of VIP receptors, could decrease the quantity of [.sup.3H] choline incorporation, microsomal choline-phosphate cytidylyltransferase (CCT) mRNA expression and CCT activity induced by VIP (10.sup.-8 mol/l) in cultured lung explants to the control levels; a VIP (10.sup.-8 mol/l) upregulated c-Fos protein expression in ATII cells. AS-ODN for c-fos oncogene (9x10.sup.-6 mol/l) could block the elevation of [.sup.3H] choline incorporation, microsomal CCT mRNA expression and CCT activity induced by VIP in cultured lung explants and in ATII cells; acents H7 (10.sup.-5 mol/l), a PKC inhibitor could also reduce VIP induced [.sup.3H] choline incorporation, microsomal CCT mRNA expression and CCT activity in cultured lung explants and in ATII cells. These results demonstrated that VIP receptors, PKC and c-Fos protein played important roles in the signaling pathway through which VIP promoted the synthesis of PC. Author Affiliation: (a) Department of Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, PR. China (b) Department of Paediatrics, First Xiangya Hospital, Central South University, Changsha 410078, PR. China Article History: Received 10 September 2006; Revised 11 November 2006; Accepted 12 November 2006