부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.molbiopara.2006.10.005 Byline: Simon Haile, Marina Cristodero, Christine Clayton, Antonio M. Estevez Keywords: Exosome; Trypanosoma brucei; RRP6 Abbreviations: RNAi, RNA interference; TAP-Tag, Tandem affinity purification tag Abstract: The exosome, a complex of 3'-exoribonucleases and associated proteins, is involved in the degradation of eukaryotic mRNAs in the cytoplasm, and has RNA processing and quality control functions in the nucleus. In yeast, the nuclear exosome differs from the cytoplasmic one in that it contains an additional non-essential component, Rrp6p. In contrast, a small proportion of human RRP6 has been shown to localise to the cytoplasm as well. When we purified the Trypanosoma brucei exosome from cytosolic extracts we found RRP6, apparently in stoichiometric amounts. We here confirm that RRP6 is in the trypanosome cytoplasm and nucleus. The level of RRP6 was unaffected by depletion of core exosome subunits by RNA interference and over-expression of tagged RRP6 was possible, indicating that RRP6 can be present independent of exosome association. Author Affiliation: ZMBH, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany Article History: Received 18 July 2006; Revised 10 October 2006; Accepted 11 October 2006