학술논문
Non-pathogenic commensal Escherichia coli bacteria can inhibit degranulation of mast cells
Document Type
Report
Author
Source
Experimental Dermatology. May, 2008, Vol. 17 Issue 5, p427, 9 p.
Subject
Language
English
ISSN
0906-6705
Abstract
To purchase or authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1600-0625.2008.00704.x Byline: Markus Magerl (1,2), Verena Lammel (2), Frank Siebenhaar (1,2), Torsten Zuberbier (1), Martin Metz (1,2), Marcus Maurer (1,2) Keywords: bacteria; degranulation; Escherichia coli; mast cells; tolerance Abstract: Abstract Background: The mast cell's (MC) ability to rapidly release presynthesized mediators allows it to play a critical role in the immune system's first-line response to pathogens. Although recent research has illuminated the role of MCs in bacterial infection, little is known about how non-pathogenic bacteria influence MC responses. Objective: To characterize the influence of non-pathogenic Escherichia coli bacteria on MCs. Results: High concentrations of live E. coli strongly down-modulated the degranulation of murine peritoneal MCs under all activator conditions. Bacteria did not induce MC degranulation by themselves, and staining of MCs showed they were still entirely viable. Dead bacteria and bacterial supernatant had no effect on MC degranulation. Ex vivo experiments confirmed that E. coli exposure down-modulates subsequently induced MC degranulation and that this effect lasts for up to a few days after exposure. Conclusion: Our results show that high doses of non-pathogenic E. coli bacteria can function as a strong, direct inhibitor of MC degranulation. This suggests a possible basis for future antiallergic treatment or prophylaxis with commensal bacteria, i.e. probiotics. Author Affiliation: (1)Department of Dermatology and Allergy, Charite- Universitatsmedizin, Berlin, Germany (2)Department of Dermatology, University Hospital Mainz, Mainz, Germany Article History: Accepted for publication 18 January 2008 Article note: Marcus Maurer, MD, Allergie-Centrum-Charite, Department of Dermatology and Allergy, Charite- Universitatmedizin, Chariteplatz 1, 10117 Berlin, Germany, Tel.: +49 30 450 518 043, Fax: +49 30 450 518 919, e-mail: marcus.maurer@charite.de