학술논문
A rapid assay for drug sensitivity of glioblastoma stem cells
Document Type
Author abstract
Source
Biochemical and Biophysical Research Communications. July 6, 2007, Vol. 358 Issue 3, p908, 6 p.
Subject
Language
English
ISSN
0006-291X
Abstract
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.bbrc.2007.05.020 Byline: Hilah Gal (a)(c)(d), Arik Makovitzki (b), Ninette Amariglio (d), Gideon Rechavi (d), Zvi Ram (e), David Givol (a) Keywords: Neurospheres; 96-Well plate; Gleevec; DAPT; Drug sensitivity Abstract: Glioblastoma (GBM) is a highly infiltrating, aggressive brain cancer with no available curative treatment. We developed a rapid assay for assessing the effect of various drugs on GBM stem cells. The assay uses a small number of separated CD133+ cells (20,000 in 0.2ml) in 96-well plate that form neurospheres within 1-2 days. Various drugs disperse the neurospheres within 24-36h, which can be quantified microscopically. We used the GBM cell line A-172 to develop the conditions for the assay, utilizing Gleevec, the gamma-secretase inhibitor DAPT, and the anti-bacterial peptide amph1D. The results show dispersion of the neurospheres leading to cell death, at relatively low drugs concentrations ( Author Affiliation: (a) Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel (b) Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel (c) Department of Physics of Complex Systems, Weizmann Institute of Science, Rehovot 76100, Israel (d) Department of Pediatric Hemato-Oncology, Chaim Sheba Medical Center, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv 52621, Israel (e) Department of Neurosurgery, Tel-Aviv Medical Center, Tel-Aviv, Israel Article History: Received 1 May 2007