부산대학교 도서관

To purchase or authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1471-4159.2006.04344.x Byline: James R. Monaghan (*), John A. Walker (*), Robert B. Page (*), Srikrishna Putta (*), Christopher K. Beachy ([dagger]), S. Randal Voss (*) Keywords: axolotl; in situ hybridization; microarray; regeneration; salamander; spinal cord Abstract: Abstract In contrast to mammals, salamanders have a remarkable ability to regenerate their spinal cord and recover full movement and function after tail amputation. To identify genes that may be associated with this greater regenerative ability, we designed an oligonucleotide microarray and profiled early gene expression during natural spinal cord regeneration in Ambystoma mexicanum. We sampled tissue at five early time points after tail amputation and identified genes that registered significant changes in mRNA abundance during the first 7 days of regeneration. A list of 1036 statistically significant genes was identified. Additional statistical and fold change criteria were applied to identify a smaller list of 360 genes that were used to describe predominant expression patterns and gene functions. Our results show that a diverse injury response is activated in concert with extracellular matrix remodeling mechanisms during the early acute phase of natural spinal cord regeneration. We also report gene expression similarities and differences between our study and studies that have profiled gene expression after spinal cord injury in rat. Our study illustrates the utility of a salamander model for identifying genes and gene functions that may enhance regenerative ability in mammals. Author Affiliation: (*)Department of Biology & Spinal Cord and Brain Injury Research Center, University of Kentucky, Lexington, Kentucky, USA ([dagger])Department of Biology, Minot State University, Minot, North Dakota, USA Article History: Received July 11, 2006; revised manuscript received August 24, 2006; accepted September 17, 2006. Article note: Address correspondence and reprint requests to S. Randal Voss Ph. D., 741 South Limestone Avenue, Lexington, KY 40536, USA. E-mail: srvoss@uky.edu