학술논문
Association between the -159C/T CD14 gene polymorphism and tuberculosis in a Korean population
Document Type
Report
Source
FEMS Immunology and Medical Microbiology. Dec, 2009, Vol. 57 Issue 3, p229, 7 p.
Subject
Language
English
ISSN
0928-8244
Abstract
To authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1574-695X.2009.00602.x Byline: Young Ae Kang (1), Hye Won Lee (1), Young Whan Kim (1), Sung Koo Han (1), Young-Soo Shim (1), Jae-Joon Yim (1) Keywords: tuberculosis; CD14; susceptibility; polymorphism Abstract: Abstract The aim of the present study was to confirm the association between the CD14-159C/T polymorphism and tuberculosis in the Korean population and to elucidate the functional basis for this putative association. CD14-159C/T genotypes were determined by PCR - restriction fragment length polymorphism analysis in 274 tuberculosis patients and 422 healthy controls. Recombinant CD14 promoter-luciferase reporter constructs, including the -159T or -159C allele, were transfected into K562 and BEAS-2B cells, and luciferase activities were measured and compared. Levels of serum sCD14 and interferon-[gamma] secreted by peripheral blood mononuclear cells (PBMCs) were measured using enzyme-linked immunosorbent assay.The frequency of -159TT genotypes was higher in tuberculosis patients than in healthy controls. The promoter activity of the -159T allele was higher than that of the -159C allele. Serum sCD14 levels were higher among tuberculosis patients with -159TT genotypes than among those with -159CC genotypes and interferon-[gamma] release by PBMCs was decreased in subjects with -159TT genotypes. In conclusion, the -159TT CD14 genotypes were associated with tuberculosis development in Koreans. This association might be a result of the higher promoter activity of the -159T allele, the higher level of sCD14, and the decreased interferon-[gamma] secretion in subjects with -159TT genotypes. Author Affiliation: (1)Department of Internal Medicine and Lung Institute, Division of Pulmonary and Critical Care Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea Article History: Received 16 June 2009; revised 6 August 2009; accepted 13 August 2009.Final version published online 15 September 2009. Article note: Correspondence: Jae-Joon Yim, Department of Internal Medicine and Lung Institute, Division of Pulmonary and Critical Care Medicine, Seoul National University College of Medicine, 101 Daehangno, Jongno-gu, Seoul 110-744, Korea. Tel.: +82 2 2072 2059; fax: +82 2 762 9662; e-mail: yimjj@snu.ac.kr