학술논문
Involvement of the GroE chaperonins in the nickel-dependent anaerobic biosynthesis of NiFe-hydrogenases of Escherichia coli
Document Type
Academic Journal
Author
Source
Journal of Bacteriology. August, 1996, Vol. 178 Issue 15-16, p4453, 8 p.
Subject
Language
ISSN
0021-9193
Abstract
The Escherichia coli chaperonins, GroES and GroEL, have a role in the biosynthesis of a functional Ni-Fe hydrogenase isoenzyme HYD3 through the incorporation of nickel. The GroES and GroEL thermosensitive mutant strains show a 60% reduction in total hydrogenase activity. A temperature shift during groE mutant growth inhibits HYD1 activity and allows accumulation of HYD3 precursor. However, processing of HYD2 and its activity remain unaffected. The nickel uptake mutant, nik, also shows a reduction in hydrogenase activity and an accumulation of precursors of HYD2 and HYD3.