학술논문
An assay for determination of rat adrenal catechol-0-methyltransferase activity: comparison of spontaneously hypertensive rats and Wistar-Kyoto rats
Document Type
Report
Author
Source
Analytical and Bioanalytical Chemistry. Dec 1, 2004, Vol. 380 Issue 7-8, p887, 4 p.
Subject
Language
English
ISSN
1618-2642
Abstract
A method has been developed for measurement of catechol-O-methyltransferase (COMT) activity in the rat adrenal gland. Epinephrine, synthesized in the adrenal gland, was used as substrate, and its enzymatic product, metanephrine, was quantified by high-performance liquid chromatography (HPLC) with fluorescence detection. The method has sufficient precision and accuracy. Soluble (S) and membrane-bound (MB) COMT activity in Wistar-Kyoto (WKY) rats was 20.7[+ or -]3.5 and 18.6[+ or -]3.4 pmol [min.sup.-1] [mg.sup.-1] protein (n = 5), respectively. To clarify the role of adrenal COMT in blood-pressure regulation, S and MB COMT activity in spontaneously hypertensive rats were determined. Respective activity was 18.6[+ or -]3.4 and 17.0[+ or -]1.1 pmol [min.sup.-1] [mg.sup.-1] (n=5), which is similar to that in WKY rats. This finding suggests that COMT in the adrenal gland might not be related to blood pressure regulation. Keywords Adrenal * Epinephrine * Catechol-O-methyltransferase * Fluorescence
Introduction The adrenal gland is an important organ which produces and releases catecholamines, especially epinephrine (E). E is secreted into the blood in response to several stresses, and acts as [...]
Introduction The adrenal gland is an important organ which produces and releases catecholamines, especially epinephrine (E). E is secreted into the blood in response to several stresses, and acts as [...]