학술논문
Comparative genomic analysis of tumors: detection of DNA losses and amplification
Document Type
Academic Journal
Author
Source
Proceedings of the National Academy of Sciences of the United States. Jan 3, 1995, Vol. 92 Issue 1, p151, 5 p. table
Subject
Language
ISSN
0027-8424
Abstract
Representational difference analysis (RDA), where human tumor cell DNA drives hybridization against normal DNA, helps detect genomic regions showing homozygous depletion in cultured cancer cells. Conversely, RDA also involves driving of hybridization by normal DNA against tumor cell DNA, isolating probes that detect amplification in tumors. Such bi-operational RDA performed on tumor DNAs isolates a probe that identifies frequent homozygous loss in colon cancer cell lines and localized to chromosome 3P.