부산대학교 도서관

To authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1365-2672.2009.04354.x Byline: M.C. Bufalo (1), A.S. Figueiredo (1), J.P.B. de Sousa (2), J.M.G. Candeias (1), J.K. Bastos (2), J.M. Sforcin (1) Keywords: antiviral action; Baccharis dracunculifolia; poliovirus; propolis; real-time PCR Abstract: Abstract Aims: The aim of this work was to evaluate the antiviral activities of Baccharis dracunculifolia (extract and essential oil), propolis and some isolated compounds (caffeic and cinnamic acids) against poliovirus type 1 (PV1) replication in HEp-2 cells. Method: Three different protocols (pre-, simultaneous and post-treatments) were used to verify the effect of addition time of the variables on PV1 replication by crystal violet method and relative viral RNA quantification by real-time PCR for analysing in which step of virus replication the variables could interfere. Conclusions: Data revealed that the B. dracunculifolia showed the best antiviral activity percentage in the simultaneous treatment, as well as lower relative viral quantification by real-time PCR. Variables might block partially the viral entry within cells, affect the steps of viral cycle replication into cells, or lead to RNA degradation before the virus entry into cells or after their release to the supernatant. Significance and Impact of the Study: Baccharis dracunculifolia is the most important botanical source of the south-eastern Brazilian propolis, and its potential for the development of new phytotherapeutic medicines has been investigated. Propolis is commonly used for its antimicrobial and immunomodulatory activities. Nevertheless, B. dracunculifolia and propolis effects on PV1 have not been investigated yet. Author Affiliation: (1) Department of Microbiology and Immunology, Biosciences Institute, UNESP, Botucatu, SP, Brazil (2) Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences of Ribeirao Preto, Laboratory of Pharmacognosy, USP, Ribeirao Preto, SP, Brazil Article History: 2009/0280: received 12 February 2009, revised 31 March 2009 and accepted 4 April 2009 Article note: Jose Mauricio Sforcin, Department of Microbiology and Immunology, Biosciences Institute, UNESP, Botucatu, SP 18618-000, Brazil. E-mail: sforcin@ibb.unesp.br