부산대학교 도서관

Background Genetic analysis of gene expression level is a promising approach for characterizing candidate genes that are involved in complex economic traits such as meat quality. In the present study, we conducted expression quantitative trait loci (eQTL) and allele-specific expression (ASE) analyses based on RNA-sequencing (RNAseq) data from the longissimus muscle of 189 Duroc x Luchuan crossed pigs in order to identify some candidate genes for meat quality traits. Results Using a genome-wide association study based on a mixed linear model, we identified 7192 cis-eQTL corresponding to 2098 cis-genes (p [less than or equai to] 1.33e-3, FDR [less than or equai to] 0.05) and 6400 trans-eQTL corresponding to 863 trans-genes (p [less than or equai to] 1.13e-6, FDR [less than or equai to] 0.05). ASE analysis using RNAseq SNPs identified 9815 significant ASE-SNPs in 2253 unique genes. Integrative analysis between the cis-eQTL and ASE target genes identified 540 common genes, including 33 genes with expression levels that were correlated with at least one meat quality trait. Among these 540 common genes, 63 have been reported previously as candidate genes for meat quality traits, such as PHKG1 (q-value = 1.67e-6 for the leading SNP in the cis-eQTL analysis), NUDT7 (q-value = 5.67e-13), FADS2 (q-value = 8.44e-5), and DGAT2 (q-value = 1.24e-3). Conclusions The present study confirmed several previously published candidate genes and identified some novel candidate genes for meat quality traits via eQTL and ASE analyses, which will be useful to prioritize candidate genes in further studies.
Author(s): Yan Liu[sup.1,2,3], Xiaolei Liu[sup.1,2,3], Zhiwei Zheng[sup.1,2,3], Tingting Ma[sup.1,2,3], Ying Liu[sup.1,2,3], Huan Long[sup.1,2,3], Huijun Cheng[sup.1,2,3], Ming Fang[sup.5], Jing Gong[sup.4], Xinyun Li[sup.1,2,3], Shuhong Zhao[sup.1,2,3] and Xuewen Xu[sup.1,2,3] Background In the past [...]