부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.bbrc.2007.06.101 Byline: Ryo Misaki (a), Takatoshi Nakagawa (b), Mitsunori Fukuda (c), Naoyuki Taniguchi (d), Tomohiko Taguchi (a) Keywords: Endocytosis; Early endosomes; Late endosomes; Lysosomes; Recycling endosomes; EGF; Transferrin; Rab5; Rab7; Rab11 Abstract: After endocytosis, most membrane proteins and lipids return to the plasma membrane (recycling pathway), but some membrane components are delivered to lysosomes (degradation pathway). These two pathways diverge in early endosomes. The recycling pathway involves recycling endosomes and the degradation pathway incorporates late endosomes and lysosomes. In many cell lines, these organelles often are located in the perinuclear region where they visually intermix. The present study, by tracking specific ligands (epidermal growth factor and transferrin) and expression of Rab proteins (Rab5, Rab7, and Rab11), demonstrated that, in COS-1 cells, the two pathways were spatially segregated. Recycling endosomes were mostly confined within the ring-shaped structure of the Golgi complex ("the Golgi ring"), whereas late endosomes and lysosomes were excluded from inside the Golgi ring. Thus, the unique organization of endocytic organelles in COS-1 cells can be utilized to visualize endocytic trafficking pathways in detail. Author Affiliation: (a) Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan (b) Department of Glycotherapeutics, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan (c) Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Miyagi 980-8578, Japan (d) Department of Disease Glycomics, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan Article History: Received 13 June 2007