부산대학교 도서관

Background: Due to their prevalence worldwide, the [beta]-lactamases CTX-M and plasmid-mediated CMY-2 are important antimicrobial resistance enzymes in a clinical setting. While culture- and PCR-based detection methods exist for these targets, they are time consuming and require specialist equipment and trained personnel to carry out. Methods: In this study, three rapid diagnostic single-plex and a prototype triplex assay were developed, using recombinase polymerase amplification with lateral flow detection (RPA-LF), and tested for their sensitivity and specificity using two isolate DNA panels (n = 90 and n = 120 isolates). In addition, the RPA-LF assays were also tested with a small number of faecal extract samples (n = 18). Results: The RPA-LF assays were able to detect [bla.sub.CXT-M-group-1], [bla.sub.CTX-M-group-9] and [bla.sub.CMY-2- type] variants with high sensitivity (82.1%-100%) and specificity (100%) within a short turnaround time (15-20 min for amplification and detection). Conclusions: RPA-LF assays developed in this study have the potential to be used at or close to the point of care, as well as in low-resource settings, producing rapid results to support healthcare professionals in their treatment decisions.
Background ESBLs afford bacteria resistance to penicillins, expanded-spectrum cephalosporins and monobactams, (1-4) and infections caused by bacteria carrying ESBLs are associated with increased morbidity and mortality. (5) The CTX-M type [...]