부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.biortech.2005.10.045 Byline: Benjamas Thanomsub (a), Wanna Pumeechockchai (a), Anirut Limtrakul (a), Panarat Arunrattiyakorn (b), Wipawan Petchleelaha (b), Teruhiko Nitoda (c), Hiroshi Kanzaki (c) Keywords: Rhamnolipid; Glycolipid; Pseudomonas; Biosurfactant; Anticancer; Biological activity Abstract: The aim of this work was to study chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste. The culture produced two biosurfactants, a and b, which showed strong activity and were identified as l-rhamnopyranosyl-l-rhamnopyranosyl-[beta]-hydroxydecanoyl-[beta]-hydroxydecanoate or Rha-Rha-C.sub.10-C.sub.10 and l-rhamnopyranosyl-l-rhamnopyranosyl-[beta]-hydroxydecanoyl-[beta]-hydroxydodecanoate or Rha-Rha-C.sub.10-C.sub.12, respectively. Both compounds exhibited higher surfactant activities tested by the drop collapse test than several artificial surfactants such as SDS and Tween 80. Rhamnolipid a showed significant antiproliferative activity against human breast cancer cell line (MCF-7) at minimum inhibitory concentration (MIC) at 6.25[mu]g/mL while rhamnolipid b showed MIC against insect cell line C6/36 at 50[mu]g/mL. Author Affiliation: (a) Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand (b) Department of Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand (c) Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University, Okayama 700-8530, Japan Article History: Received 18 April 2005; Revised 20 October 2005; Accepted 24 October 2005