부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.jep.2005.09.006 Byline: C. Penido (a), K.A. Costa (a), D.O. Futuro (b), S.R. Paiva (c), M.A.C. Kaplan (d), M.R. Figueiredo (c), M.G.M.O. Henriques (a) Keywords: Stachytarpheta cayennensis; Verbenaceae; Ipolamiide; Verbascoside; Anti-inflammatory; Anti-ulcerogenic Abstract: In the present work, the anti-inflammatory and gastroprotective properties of ethanolic extracts of Stachytarpheta cayennesis (L.C. Rich) Vahl (Verbenaceae) were assessed. Chromatographic analysis of the crude ethanolic extract, SC01, revealed high concentrations of the iridoid ipolamiide, whereas the SC02, the second ethanolic extract, presented the arylpropanoid verbacoside as a major constituent. The oral administration of SC01 (100mg/kg) into Swiss mice failed to inhibit paw oedema and pleural exudation induced by carrageenan and zymosan, whereas SC02 (100mg/kg, p.o.) inhibited oedema and protein extravasation in all instances. Both extracts inhibited total leukocyte accumulation into the pleural cavity 4 and 24h after the intrathoracic (i.t.) injection of carrageenan, due to the inhibition of neutrophil and mononuclear cell influx, whereas only SC02 was able to inhibit leukocyte mobilization induced by zymosan (100[mu]g/cavity, i.t.). SC02 inhibited LPS (250ng/cavity)-induced total leukocyte, neutrophil and eosinophil accumulation in the pleural cavity, whereas SC01 selectively inhibited neutrophil influx. In addition, our data indicates that the extract SC02 presents an important anti-ulcerogenic activity, since it inhibited diclofenac-induced (100mg/kg, p.o.) gastric ulcera. Overall, these data provide evidence for the anti-inflammatory and gastroprotective properties of Stachytarpheta cayennensis, supporting its use in folk medicine for such purposes. Author Affiliation: (a) Departamento de Farmacologia Aplicada, Far-Manguinhos, Fundacao Oswaldo Cruz, Rua Sizenando Nabuco 100, 21041-250 Rio de Janeiro, RJ, Brazil (b) Departamento de Tecnologia FarmacA*utica e de Cosmeticos, Faculdade de Farmacia, Universidade Federal Fluminense, Rua MarquA*s de Parana 282, 24030-221 Niteroi, RJ, Brazil (c) Departamento de Quimica de Produtos Naturais III, Far-Manguinhos, Fundacao Oswaldo Cruz, Rua Sizenando Nabuco 100, 21041-250 Rio de Janeiro, RJ, Brazil (d) Nucleo de Pesquisa de Produtos Naturais, Universidade Federal do Rio de Janeiro, Cidade Universitaria, Rio de Janeiro, RJ, Brazil Article History: Received 12 April 2005; Revised 23 August 2005; Accepted 2 September 2005