학술논문
Hepatoprotective activity of chrysin is mediated through TNF-[alpha] in chemically-induced acute liver damage: an in vivo study and molecular modeling
tumor necrosis factor
tumor necrosis factor
Document Type
Report
Author
Source
Experimental and Therapeutic Medicine. May 2017, Vol. 13 Issue 5, p1671, 10 p.
Subject
Language
English
ISSN
1792-0981
Abstract
Introduction The liver is the primary organ responsible for the metabolism of numerous xenobiotics, including drugs and toxic chemicals (1). Carbon tetrachloride (C[Cl.sub.4]), an industrial solvent, cleaner and degreaser, has [...]
Chrysin (5,7-dihydroxyflavone) is a naturally occurring flavonoid present at high levels in honey, propolis and numerous plant extracts. Chrysin is known to have hepatoprotective activity, however, the mechanisms by which it exerts this effect remain unclear. In the present study, the effects of chrysin in carbon tetrachloride (C[Cl.sub.4])-induced acute liver damage were investigated and the results used to infer a possible mechanism behind chrysin's hepatoprotective activity. Prior to an intraperitoneal injection of C[Cl.sub.4] (1 ml/kg) to induce acute liver damage, chrysin (50 mg/kg) was administered orally to mice for 7 days. The positive control group was given 50 mg/kg standardized silymarin, a well-studied hepatoprotective flavonoid. Twenty-four h following C[Cl.sub.4] administration, an increase in the activity levels of serum aspartate-amino-transferase and alanine-amino-transferase was found. This was accompanied by extended centrilobular necrosis, steatosis and an altered hepatocyte ultrastructure. In addition, C[Cl.sub.4]-induced acute hepatotoxicity was associated with an increase in hepatic tumor necrosis factor-[alpha] (TNF-[alpha]) and a-smooth muscle actin ([alpha]-SMA) protein expression, which was significantly decreased in the livers of mice pre-treated with chrysin (P Key words: chrysin, liver, hepatoprotection, tumor necrosis factor-[alpha], molecular modeling, carbon tetrachloride, a-smooth muscle actin, silymarin
Chrysin (5,7-dihydroxyflavone) is a naturally occurring flavonoid present at high levels in honey, propolis and numerous plant extracts. Chrysin is known to have hepatoprotective activity, however, the mechanisms by which it exerts this effect remain unclear. In the present study, the effects of chrysin in carbon tetrachloride (C[Cl.sub.4])-induced acute liver damage were investigated and the results used to infer a possible mechanism behind chrysin's hepatoprotective activity. Prior to an intraperitoneal injection of C[Cl.sub.4] (1 ml/kg) to induce acute liver damage, chrysin (50 mg/kg) was administered orally to mice for 7 days. The positive control group was given 50 mg/kg standardized silymarin, a well-studied hepatoprotective flavonoid. Twenty-four h following C[Cl.sub.4] administration, an increase in the activity levels of serum aspartate-amino-transferase and alanine-amino-transferase was found. This was accompanied by extended centrilobular necrosis, steatosis and an altered hepatocyte ultrastructure. In addition, C[Cl.sub.4]-induced acute hepatotoxicity was associated with an increase in hepatic tumor necrosis factor-[alpha] (TNF-[alpha]) and a-smooth muscle actin ([alpha]-SMA) protein expression, which was significantly decreased in the livers of mice pre-treated with chrysin (P Key words: chrysin, liver, hepatoprotection, tumor necrosis factor-[alpha], molecular modeling, carbon tetrachloride, a-smooth muscle actin, silymarin