학술논문
Methylene Blue Metabolic Therapy Restrains In Vivo Ovarian Tumor Growth
Document Type
Academic Journal
Author
Source
Cancers. January 2024, Vol. 16 Issue 2
Subject
Language
English
ISSN
2072-6694
Abstract
Author(s): Jorgelindo da Veiga Moreira (corresponding author) [1,*]; Nancy Nleme [1]; Laurent Schwartz [2]; Kim Leclerc-Desaulniers [3]; Euridice Carmona [3]; Anne-Marie Mes-Masson [3,4]; Mario Jolicoeur (corresponding author) [1,*] 1. Introduction [...]
This study confirms the potential of methylene blue (MB) in treatment strategies for ovarian cancer. Ovarian cancer cells taken from patients were inserted into mice, and their growth was followed together with mouse health. Our findings revealed MB’s ability to impede ovarian cancer progression, especially against tumors showing resistance to conventional chemotherapies. By investigating the effects of MB on cancer cell behavior and metabolism, this research revealed novel therapeutic targets for affecting tumor growth. These insights suggest that MB could be a valuable complementary therapeutic to existing treatments or an alternative for patients facing limited options. This research thus opens new avenues for developing targeted therapies that can remotely control cancer cell development. Ovarian cancer remains a significant challenge, especially in platinum-resistant cases where treatment options are limited. In this study, we investigated the potential of methylene blue (MB) as a metabolic therapy and complementary treatment approach for ovarian cancer. Our findings demonstrated a significant in vivo reduction in the proliferation of TOV112D-based ovarian-cell-line xenografts. In this preclinical study, which used a carboplatin-resistant ovarian cancer tumor model implanted into mice, MB-mediated metabolic therapy exhibited superior tumor slowdown compared to carboplatin treatment alone. This indicates, for the first time, MB’s potential as an alternative or adjuvant treatment, especially for resistant cases. Our in vitro study on TOV112D and ARPE-19 sheds light on the impact of such an MB-based metabolic therapy on mitochondrial energetics (respiration and membrane potential). MB showed a modulatory role in the oxygen consumption rate and the mitochondrial membrane potential. These results revealed, for the first time, that MB specifically targets TOV112D mitochondria and probably induces cell apoptosis. The differential response of normal (ARPE-19) and cancer (TOV112D) cells to the MB treatment suggests potential alterations in cancer cell mitochondria, opening avenues for therapeutic approaches that target the mitochondria. Overall, our findings suggest the efficacy of MB as a possible treatment for ovarian cancer and provide valuable insights into the mechanisms underlying the efficacy of methylene blue metabolic therapy in ovarian cancer treatment.
This study confirms the potential of methylene blue (MB) in treatment strategies for ovarian cancer. Ovarian cancer cells taken from patients were inserted into mice, and their growth was followed together with mouse health. Our findings revealed MB’s ability to impede ovarian cancer progression, especially against tumors showing resistance to conventional chemotherapies. By investigating the effects of MB on cancer cell behavior and metabolism, this research revealed novel therapeutic targets for affecting tumor growth. These insights suggest that MB could be a valuable complementary therapeutic to existing treatments or an alternative for patients facing limited options. This research thus opens new avenues for developing targeted therapies that can remotely control cancer cell development. Ovarian cancer remains a significant challenge, especially in platinum-resistant cases where treatment options are limited. In this study, we investigated the potential of methylene blue (MB) as a metabolic therapy and complementary treatment approach for ovarian cancer. Our findings demonstrated a significant in vivo reduction in the proliferation of TOV112D-based ovarian-cell-line xenografts. In this preclinical study, which used a carboplatin-resistant ovarian cancer tumor model implanted into mice, MB-mediated metabolic therapy exhibited superior tumor slowdown compared to carboplatin treatment alone. This indicates, for the first time, MB’s potential as an alternative or adjuvant treatment, especially for resistant cases. Our in vitro study on TOV112D and ARPE-19 sheds light on the impact of such an MB-based metabolic therapy on mitochondrial energetics (respiration and membrane potential). MB showed a modulatory role in the oxygen consumption rate and the mitochondrial membrane potential. These results revealed, for the first time, that MB specifically targets TOV112D mitochondria and probably induces cell apoptosis. The differential response of normal (ARPE-19) and cancer (TOV112D) cells to the MB treatment suggests potential alterations in cancer cell mitochondria, opening avenues for therapeutic approaches that target the mitochondria. Overall, our findings suggest the efficacy of MB as a possible treatment for ovarian cancer and provide valuable insights into the mechanisms underlying the efficacy of methylene blue metabolic therapy in ovarian cancer treatment.