부산대학교 도서관

To authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1365-2052.2009.01857.x Byline: C. H. Wu (*), W. Jin ([dagger]), K. Nomura ([double dagger]), T. Goldammer (s.), T. Hadfield (*), B. P. Dalrymple ([paragraph]), S. McWilliam ([paragraph]), J. F. Maddox (**), N. E. Cockett (*) Keywords: chromosome; comparative map; ovine; radiation hybrid; virtual sheep genome Abstract: Summary Ovis aries chromosome one (OAR1) is the largest submetacentric chromosome in the sheep genome and is homologous to regions on human chromosomes 1, 2, 3 and 21. Using the USUoRH5000 ovine whole-genome radiation hybrid (RH) panel, we have constructed a RH map of OAR1 comprising 102 framework and 75 placed/binned markers across five linkage groups spanning 3759.43 cR5000, with an average marker density of 21.2 cR5000/marker. The alignment of our OAR1 RH map shows good concordance with the recently developed virtual sheep genome, with fewer than 1.86% discrepancies. A comparative map of OAR1 was constructed by examining the location of RH-mapped orthologues in sheep within the genomes of cow, human, horse and dog. Analysis of the comparative map indicates that conserved syntenies within the five ovine RH linkage groups underwent internal chromosomal rearrangements which, in general, reflect the evolutionary distances between sheep and each of these four species. The ovine RH map presented here integrates all available mapping data and includes new genomic information for ovine chromosome 1. Author Affiliation: (*)Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, UT 84322-4700, USA ([dagger])Laboratory for Conservation and Utilization of Bio-resource, Yunnan University, Kunming 650091, China ([double dagger])Laboratory of Animal Genetics and Breeding, Department of Animal Science, Tokyo University of Agriculture, 1737 Funako Atsugi-shi, Kanagawa 243-0034, Japan (s.)Research Unit for Molecular Biology, Research Institute for the Biology of Farm Animals (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany ([paragraph])CSIRO Livestock Industries, Queensland Bioscience Precinct, St Lucia, Qld 4067, Australia (**)Department of Veterinary Science, University of Melbourne, Vic. 3010, Australia Article History: Accepted for publication 8 December 2008 Article note: N. E. Cockett, Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 4900 Old Main Hill, Logan, UT 84322-4900, USA., E-mail: noelle.cockett@usu.edu