부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.lfs.2007.01.036 Byline: M.T. Lindenmeyer (a), C. Kern (a), T. Sparna (a), J. Donauer (b), J. Wilpert (b), J. Schwager (c), D. Porath (c), C. Kreutz (d), J. Timmer (d), I. Merfort (a) Keywords: Sesquiterpene lactones; Parthenolide; Gene transcription; cDNA microarray; Real-time quantitative PCR Abstract: Sesquiterpene lactones are known for their anti-inflammatory activity which has been proven in various assays on DNA, mRNA and protein level. Here we report on the change in the gene expression profile in TNF-[alpha] stimulated human 293 cells after treatment with parthenolide using a cDNA microarray analysis. Twenty-one of 7028 genes were found to be up- and 18 down-regulated. They encode for chemoattractants, immune system proteins, glycoproteins, metabolism, serine proteinases, and transcription factors. Confirmatory analyses were carried out using quantitative real-time RT-PCR (TaqMan.sup.[R]). Additional studies with selected genes revealed the concentration-dependent influence of parthenolide on the expression of these genes. Author Affiliation: (a) Institute for Pharmaceutical Sciences, Department of Pharmaceutical Biology and Biotechnology, Albert-Ludwigs-University, Freiburg, Germany (b) Renal Division, Department of Medicine, University Hospital Freiburg, Germany (c) DSM Nutritional Products 4303 Kaiseraugst, Switzerland (d) Center for Data Analysis and Modeling, Albert-Ludwigs-University, Freiburg, 79104 Freiburg, Germany Article History: Received 13 August 2006; Accepted 20 January 2007