부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.exer.2007.01.021 Byline: S.Y. Ghoghawala (a), M.J. Mannis (b), C.J. Murphy (e), M.I. Rosenblatt (b), R.R. Isseroff (c)(d) Abstract: An optimal system for monitoring in vivo corneal wound healing is inexpensive, has utility for wounding and imaging, and is able to provide previews before photography. We outline such an imaging system that takes advantage of a consumer digital camera and an LED-based light source for fluorescein excitation. Using FVB/NJ mice, 2mm diameter, circular, axial corneal epithelial defects were created using a crescent blade. The corneal wounds were imaged every four hours until healed using a Nikon Coolpix 5400 camera attached to a Nikon SMZ-10A stereomicroscope, using the illumination from a 16 LED 464nm flashlight. The wound area was calculated, and the linear regressions of the linear phase of wound healing were compared using the F-test. The slopes of the linear regressions for the 6 trials of 4 mice/trial had an average of -52.95[mu]m/h (SEM=0.55[mu]m/h) and were statistically equivalent (p 0.05). The mean of the R.sup.2 values for the linear regressions was 0.9546 (SEM=0.0121). The equivalent linear regressions and R.sup.2 0.90 suggest that the imaging system could precisely monitor the wound healing of multiple trials and of animals within each trial, respectively. Using a consumer digital camera and LED-based illumination, we have established a system that is economical, is used in both wounding and imaging, is operated by a single person, and is able to provide real-time previews to monitor corneal wound healing precisely. Author Affiliation: (a) School of Medicine, University of California-Davis, One Shields Avenue, TB 192, Davis, CA 95616, USA (b) Ophthalmology and Vision Science, University of California-Davis, 4860 Y Street, Suite 2400, Sacramento, CA 95817, USA (c) Department of Dermatology, University of California-Davis, One Shields Avenue, TB 192, Davis, CA 95616, USA (d) Dermatology Service, Northern California Health Care System, Department of Veterans Affairs, Mather, CA 95655, USA (e) Schools of Veterinary Medicine, Medicine and Engineering, University of Wisconsin-Madison, USA Article History: Received 27 September 2006; Accepted 12 January 2007 Article Note: (footnote) [star] Grant Information: This research was supported in part by a UC Davis Predoctoral Research Fellowship (to SYG) and by grants from the NIH-NIAMS (AR44518 to RRI), the NIH-NEI (K08EY015829 to MIR and RO1EY012253-05 to CJM), the NIH-NHLBI (1R01HL079012-01A1 to CJM) and Research to Prevent Blindness, Inc. (to MJM).