부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.actbio.2008.04.011 Byline: Feng Chai (a), Nicolas Mathis (b), Nicolas Blanchemain (a), Cathy Meunier (b), Hartmut F. Hildebrand (a) Keywords: DLC coatings; Surface energy; Osteoblasts response; Adhesion; Cytoskeleton Abstract: Diamond-like carbon (DLC) coating is a convenient means of modifying material surfaces that are sensitive to wear, such as titanium and silica substrates. This work aims to evaluate the osteoblast-like cells' response to DLC-coated Si (Si-DLC), which was treated under different conditions. DLC and deuterated DLC films were deposited by plasma-enhanced chemical vapor deposition to obtain a 200-nm-thick layer on all the samples. Three types of precursor gas were applied for deposition: pure methane (CH.sub.4), pure deuterated methane (CD.sub.4) and their half/half mixture. All surface treatments were performed under two different self-bias voltages (V.sub.sb): -400 and -600V. The modified surfaces were characterized by X-ray photoelectron spectroscopy, Raman spectroscopy, Rutherford backscattering spectroscopy, elastic recoil detection analysis, X-ray reflectometry and the sessile-drop method. MC3T3-E1 osteoblasts were cultured on the Si-DLC wafers for 3 and 6 days. Biological tests to measure cell proliferation, cell vitality, cell morphology and cell adhesion were performed. All DLC coatings produced a slightly more hydrophobic state than non-treated Si. Certain types of amorphous DLC coating, such as the surface treated under the V.sub.sb of -600V in pure methane (600CH.sub.4) or in pure deuterated methane (600CD.sub.4), offered a significantly higher cell proliferation rate to Si substrate. Scanning electron microscopy observations confirmed that the optimal cell adhesion behavior, among all the treated surfaces, occurred on the surface of the 600CH.sub.4 and 600CD.sub.4 groups, which showed increased amounts of filopodia and microvilli to enhance cell-environment exchange. In conclusion, DLC coating on Si could produce better surface stability and improved cellular responses. Author Affiliation: (a) Groupe de Recherche sur les Biomateriaux (GRB), Laboratoire de Biophysique, UPRES EA 1049, Faculte de Medecine, Universite de Lille-2, 1 place de Verdun, 59045 Lille cedex, France (b) FEMTO-ST/CREST, UMR 6174 CNRS, 4 place Tharradin, BP 71427, 25211 Montbeliard cedex, France Article History: Received 12 October 2007; Revised 23 March 2008; Accepted 4 April 2008 Article Note: (footnote) [star] Research presented at the Materials Today Asia 2007 Conference.