부산대학교 도서관

To purchase or authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1365-3083.2006.01824.x Byline: O. Parkash (*), A. Kumar ([dagger]), A. Nigam ([double dagger]), K. L. M. C. Franken (s.), T. H. M. Ottenhoff (s.) Abstract: Abstract The potential of the recombinant serine-rich 45-kDa antigen (ML0411) of Mycobacterium leprae to aid in detecting M. leprae-specific serum antibodies was assessed by an enzyme-linked immunosorbent assay (ELISA) in leprosy patients and controls comprising of tuberculosis patients, other unrelated skin-diseased patients and healthy individuals from India. All 18 multibacillary (MB) and 18/38 (47.4%) of the paucibacillary (PB) leprosy patients were found positive. None of the controls was positive, yielding complete (0/49) specificity in the series tested here. On the other hand, an anti-phenolic glycolipid-1 (PGL-I) antibody-detecting assay yielded detectable responses in 94.4% (17/18) of MB and 36.8% (14/38) of PB leprosy patients. Only two of 49 (4.1%) controls were positive, giving a specificity of 95.9%. Further, there was a good concordance (agreement of 83.8%; [chi].sup.2 = 40.3, P < 0.001; [kappa] = 0.63) between the two assays. Thus, the 45-kDa-based assay was slightly better than anti-PGL-I antibody-detecting assay. Interestingly, when combining the results of both the assays together for all leprosy patients (MB + PB), the combined sensitivity was significantly higher than that of the anti-PGL-I antibody-detecting ELISA alone (73.2% versus 55.4%; P 0.05) compared with the 45-kDa antigen-based assay alone. Similarly, in case of PB patients, using both assays in combination, the sensitivity was significantly higher compared with anti-PGL-I antibody-detecting assay alone (60.5% versus 36.8%; P 95%). In conclusion, the results of the present study indicate that the M. leprae 45-kDa protein is a potent B-cell antigen and may be a useful serodiagnostic reagent. Author Affiliation: (*)National JALMA Institute for Leprosy and other Mycobacterial Diseases, TajGanj, Agra 1 ([dagger])Department of Biomedical Sciences, Bundelkhand University, Jhansi ([double dagger])Department of Biochemistry, School of Life Sciences, Dr B.R. Ambedkar University, Agra, India (s.)Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, The Netherlands Article History: Received 4 May 2006; Accepted in revised form 15 June 2006 Article note: O. Parkash, National JALMA Institute for Leprosy and other Mycobacterial Diseases, TajGanj, Agra 1, India. E-mail: om1234@gmail.com