부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.ijantimicag.2009.03.023 Byline: Leyla Boumghar-Bourtchai (a), Hubert Chardon (b), Brigitte Malbruny (a)(c), Senda Mezghani (a), Roland Leclercq (a)(c), Anne Dhalluin (a)(d) Keywords: 23S ribosomal RNA; Macrolides; Azalides; Antibiotic resistance Abstract: The genetic basis of erythromycin resistance in Turicella otitidis, a coryneform bacteria associated with otitis, was studied in five macrolide-resistant clinical isolates. Macrolide resistance genes were searched for by polymerase chain reaction (PCR). Genes for domain V of 23S rRNA (rrl) as well as rplD (L4 protein) and rplV (L22 protein) genes were characterised, amplified by PCR from total genomic DNA and sequenced. In the resistant isolates, cross-resistance to macrolides and clindamycin was associated with mutations at positions 2058 and/or 2059 (Escherichia coli numbering). Three isolates displayed A2058 mutations, one isolate had an A2059G mutation whereas another one contained mutations at positions 2058 and 2059. Southern blot experiments revealed that T. otitidis had three copies of the rrl gene. In conclusion, resistance to macrolides in T. otitidis is due, at least in part, to mutations in the rrl gene. Author Affiliation: (a) EA 2128 Interactions hA[acute accent]te et microorganismes des epitheliums, University of Caen, Caen, France (b) CH du pays d'Aix, Aix, France (c) CHU CA[acute accent]te de Nacre and University of Caen, Caen, France (d) UFR des Sciences Pharmaceutiques, University of Caen, Caen, France Article History: Received 11 February 2009; Accepted 30 March 2009